Degradation of intestinal mRNA: A matter of treatment

Figure 1 Time course of degradation effect on muscle and submucosal/mucosal human and rat tissue. Human and rat gut samples were stored in MEM-Hepes on ice and separated in smooth muscle and submucosal/mucosal layer (muscle, mucosa) at different points in time. RNA from the tissues was isolated and degradation (RIN) of the different samples was examined. A: The figure shows a typical gel for human and rodent samples with different integrities. First the ladder, followed by 3 human muscle samples (hu MP), another 3 samples from human mucous layer (hu SMP) all from different points in time. The last 6 samples were comparable samples from rat (ra); B and C: Typical electrophoretic RNA measurements obtained from an Agilent 2100 bioanalyzer displaying different RIN values for intact (8 and 3 respectively; B) and partly degraded RNA (5 and 2 respectively; C); RIN values from both rat and human tissues from different compartment and time points are depicted in (D) and (E). Data are presented as the mean ± SE. n = 10.