FoxM1 overexpression promotes epithelial-mesenchymal transition and metastasis of hepatocellular carcinoma

Figure 1 Forkhead box protein M1 overexpression correlates significantly with hepatocellular carcinoma metastasis. A: Immunohistochemistry was used to detect forkhead box protein M1 (FoxM1) expression in normal liver, para-carcinoma, and carcinoma (HCC) tissues (^aP < 0.05 vs carcinoma; ^cP < 0.05 vs para-carcinoma); B: Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of FoxM1 mRNA expression in normal liver, para-carcinoma, and carcinoma (HCC) tissues (^aP < 0.05 vs carcinoma; ^cP < 0.05 vs para-carcinoma). Glyceraldehyde-3-phospate dehydrogenase (GAPDH) mRNA was used for normalization; C: Western blot analysis of FoxM1 expression in human HCC cell lines (^aP < 0.05 vs HepG2; ^cP < 0.05 vs SK-Hep1; ^eP < 0.05 vs MHCC-97H); D: qRT-PCR analysis of FoxM1 mRNA expression in human HCC cell lines (^aP < 0.05 vs HepG2; ^cP < 0.05 vs SK-Hep1; ^eP < 0.05 vs MHCC-97H). GAPDH mRNA was used for normalization; E and F: Kaplan-Meier analysis of the correlation between FoxM1 expression level and recurrence or overall survival of HCC patients.