Chloride intracellular channel 1 regulates colon cancer cell migration and invasion through ROS/ERK pathway

Figure 4 MEK/ERK/MMPs pathway involved in the metastasis of LOVO cells under hypoxia-reoxygenation conditions. A, C: Treatment with DPI (15 μmol/L), NAC (30 mmol/L) or PD98059 (50 μmol/L) decreased the protein expression of p-ERK or MMP-2 and MMP-9 in LOVO cells compared with H-R group, respectively; B, D: Treatment with 20 and 40 μmol/L IAA94 also decreased the expression of p-ERK or the protein expression of MMP-2 and MMP-9 in LOVO cells, respectively. Results were normalized to GAPDH. Values represent the mean ± SD from three independent experiments. ^bP < 0.01 vs N group, ^dP < 0.01 vs hypoxia-reoxygenation (H-R) group.