Chloride intracellular channel 1 regulates colon cancer cell migration and invasion through ROS/ERK pathway

Figure 2 Effect of hypoxia-reoxygenation on the mRNA and protein expression of CLIC1 and wound healing assays in LOVO cells. A, B: mRNA (A) and protein (B) expression of CLIC1 was significantly increased under hypoxia-reoxygenation (H-R) conditions as revealed by RT-PCR or Western blot analysis, respectively. Results were normalized to GAPDH; C, D: Wound recovery (%) of LOVO cells treated with DPI (15 μmol/L), NAC (30 mmol/L), PD98059 (50 μmol/L) or with IAA94 at various concentrations (1, 20 and 40 μmol/L) for 24 h under H-R conditions, respectively. Values represent the mean ± SD from three independent experiments. ^aP < 0.05, ^bP < 0.01 vs N group, ^cP < 0.05, ^dP < 0.01 vs H-R group. DPI: Diphenyleneiodonium; RT-PCR: Reverse transcription-polymerase chain reaction.