Chloride intracellular channel 1 regulates colon cancer cell migration and invasion through ROS/ERK pathway

Figure 1 Increased reactive oxygen species production in LOVO cells under hypoxia-reoxygenation conditions. LOVO cells were cultured in normoxia (N) for 24 h or under hypoxia for 4 h followed by reoxygenation for 20 h (hypoxia-reoxygenation, H-R). DPI (15 μmol/L), NAC (30 mmol/L) (A) and IAA94 at various concentration (1, 20, and 40 μmol/L) (B) decreased the reactive oxygen species production under H-R conditions. Results are expressed as fold of normoxia. Values represent the mean ± SD from three independent experiments. ^bP < 0.01 vs N group, ^dP < 0.01 vs H-R group. ROS: Reactive oxygen species; DPI: Diphenyleneiodonium; NAC: N-acetylcysteine.