Impaired balance of T helper 17/T regulatory cells in carbon tetrachloride-induced liver fibrosis in mice

Figure 5 α-Smooth muscle actin expression after different treatment. A and B: Western blotting and quantitative analysis of α-SMA; C-E: Immunofluorescence staining (C: HSCs; D: HSCs + Th17 cells; E: HSCs + Treg cells. Original magnification, × 200); F: Fluorescence intensity of α-SMA; G and I: Western blotting and quantitative analysis after HSCs treated with different numbers of Th17 cells; H and J: Western blotting and quantitative analysis after HSCs treated with different numbers of Treg cells. ^aP > 0.05 vs control, ^cP < 0.05 vs control; ^eP < 0.05 vs 1 × 10⁴ cells, P > 0.05 vs 2 × 10⁴ cells. α-SMA: α-Smooth muscle actin; HSCs: Hepatic stellate cells; Th17: T helper 17; Treg: Th17/T regulatory.