Diet high in fructose leads to an overexpression of lipocalin-2 in rat fatty liver

Figure 2 Changes in lipocalin-2 mRNA and protein expression in rats livers. A: Bar plots show relative specific mRNA levels in hepatic samples. Each bar represents as mean ± SEM of 4 rats/group at weeks 4 and 8. Data were normalized by ubiquitin C. β-actin (Actb) was used as an additional reference housekeeping gene, yielding comparable results; B: Photographs of end-point polymerase chain reaction products run on 1.1% agarose gel electrophoresis for hepatic Lcn-2 and Actb; C: Immunoblots of hepatic LCN-2 (two representative animals per group). ^bP < 0.01. Co: Chow pellets; LDC: Lieber-DeCarli liquid diet; L-HFr: LDC combined with 70% fructose.