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©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Feb 21, 2014; 20(7): 1822-1832
Published online Feb 21, 2014. doi: 10.3748/wjg.v20.i7.1822
Published online Feb 21, 2014. doi: 10.3748/wjg.v20.i7.1822
Table 1 Primers used for detection of gene transcription
| Gene | Forward | Reverse |
| α-SMA | 5'-ACTGGGACGACATGGAAAAG-3' | 5'-CATCTCCAGAGTCCAGCACA-3' |
| Pro-collagen I | 5'-AGGCATAAAGGGTCATCGTG-3' | 5'-ACCGTTGAGTCCATCTTTGC-3' |
| Collagen III | 5'-GTCCACGAGGTGACAAAGGT-3' | 5'-CATCTTTTCCAGGAGGTCCA-3' |
| TNFα | 5'-AGATGTGGAACTGGCAGAGG-3' | 5'-CCCATTTGGGAACTTCTCCT-3' |
| IL-6 | 5'-CCGGAGAGGAGACTTCACAG-3' | 5'-ACAGTGCATCATCGCTGTTC-3' |
| TGFβ1 | 5'-ATACGCCTGAGTGGCTGTCT-3' | 5'-TGGGACTGATCCCATTGATT-3' |
| MCP-1 | 5'-ATGCAGTTAATGCCCCACTC-3' | 5'-TTCCTTATTGGGGTCAGCAC-3' |
| TLR4 | 5'-TGCTCAGACATGGCAGTTTC-3' | 5'-TCAAGGCTTTTCCATCCAAC-3' |
| Myd88 | 5'-GAGATCCGCGAGTTTGAGAC-3' | 5'-CTGTTTCTGCTGGTTGCGTA-3' |
| TRAF6 | 5'-AGGGTACAATACGCCTCACG-3' | 5'-GCGGGTAGAGACTTCACAGC-3' |
| ERK1 | 5'-TCCAAGGGCTACACCAAATC-3' | 5'-AGGTAGTTTCGGGCCTTCAT-3' |
| JNK1 | 5'-GCCACAAAATCCTCTTTCCA-3' | 5'-CACATCGGGGAACAGTTTCT-3' |
| Cyclin D1 | 5'-GCGTACCCTGACACCAATCT-3' | 5'-GGCTCCAGAGACAAGAAACG-3' |
| β-actin | 5'-GCCAACACAGTGCTGTCTGG-3' | 5'-TGATCCACATCTGCTGGAAGG-3' |
- Citation: Qian H, Shi J, Fan TT, Lv J, Chen SW, Song CY, Zheng ZW, Xie WF, Chen YX. Sophocarpine attenuates liver fibrosis by inhibiting the TLR4 signaling pathway in rats. World J Gastroenterol 2014; 20(7): 1822-1832
- URL: https://www.wjgnet.com/1007-9327/full/v20/i7/1822.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i7.1822