Regulation of hepatic EAAT-2 glutamate transporter expression in human liver cholestasis

Figure 6 GLT-1/EAAT2 transporter expression in rat liver after bile duct ligation. A: Immunoblot analysis of multidrug resistance protein 2 transporter and actin protein expression in the liver of sham-operated (control, n = 4) and bile duct ligated (BDL, n = 4) rats. Fifty µg of total protein extract were loaded and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoblots are representative of at least three independent experiments; B: Immunohistochemical localization of the GLT-1/EAAT2 transporter in liver sections of sham operated (B1, B3) and bile duct ligated (B2, B4) rats. Immunohistochemistry was performed on paraffin-embedded liver sections using specific antibody for GLT-1 as described in Methods. (B1 and B3) in sham-operated liver rats, no GLT-1 immunoreactivity is observed in hepatocytes. After BDL, (B2 and B4) GLT-1 immunoreactivity is detected as a specific cell surface staining in hepatocytes [original magnification × 200 (B1, B2) and × 400 (B3, B4) respectively].