Regulation of hepatic EAAT-2 glutamate transporter expression in human liver cholestasis

Figure 2 Modulation of aspartate uptake capacity by phorbol 12-myristate 13-acetate in HepG2 cells. A: Saturation curve for D-[³H]-aspartate uptake measured in HepG2 cells treated for 15 min with 500 nmol/L phorbol 12-myristate 13-acetate (PMA) (squares) or vehicle (circles). Data shown correspond to mean values ± SE of typical experiments performed four times in quadruplicate; B: Effect of pre-treating HepG2 cells with increasing concentrations of PMA for 15 min on D-[³H]-aspartate uptake. Data are expressed as percent of control and correspond to mean ± SE of three independent experiments performed in quadruplicate; C: modulation of D-[³H]-aspartate uptake on HepG2 cells after incubation with 500 nmol/L PMA for different periods of time. Results are expressed as percent of control (non treated cells) and correspond to means ± SE of three independent experiments performed in quadruplicate. Inset shows the data obtained after the analysis of intracellular ATP levels in HepG2 cells after treatment with 500 nmol/L PMA for 1 h. Data shown correspond to mean values ± SE of typical experiments performed four times in quadruplicate.