Oleanolic acid and ursolic acid inhibit proliferation in transformed rat hepatic oval cells

Figure 1 H₂O₂ promoted WB-F344 cell proliferation. A: The effect of H₂O₂ on cell proliferation. WB-F344 cells were exposed to 7 × 10^-4-7 × 10^-9 mol/L H₂O₂ for 6, 9, 12, 15, and 18 h. Cell proliferation was evaluated using the MTT assay. The data represent the mean ± SD derived from three independent experiments; B: Flow cytometric analyses of the cell cycle in H₂O₂-treated WB-F344 cells. The cells in the control and the H₂O₂ groups were stained with 10 μg/mL propidium iodide, and the DNA content was analyzed as described in the Materials and Methods; C: Histograms indicating the percentages of WB-F344 cells in the control and H₂O₂ exposure groups in the G₁, G₂/M and S phases. The data represent the mean ± SD derived from three independent experiments. ^aP < 0.05 vs control group.