Novel methylxanthine derivative-mediated anti-inflammatory effects in inflammatory bowel disease

Figure 2 Caffeine, pentoxifylline and theophylline suppress protein kinase B signaling pathway activation in mouse colonic epithelial cells. CMT93 mouse colonic epithelial cells were stimulated with caffeine (CAF) (0, 1, 2.5 or 5 mmol/L), pentoxifylline (PTX) or theophylline (THEO) (0, 10, 50 or 100 mmol/L) for 48 h. Twenty five micro grams of total protein were resolved using SDS-polyacrylamide gel electrophoresis and analyzed by Western blot using anti-phospho/total protein kinase B (AKT) Abs purchased from cell signaling technology (Danvers, MA, United States) .