Increased expression of tumor necrosis factor-α is associated with advanced colorectal cancer stages

Figure 3 Semi-quantitative evaluation of tumor necrosis factor-α protein expression. A: Percentage of samples showing no staining, weak or strong immunohistochemistry staining; B, D and E: Representative microscopic images of tumor necrosis factor-α (TNF-α) immunohistochemical expression of by tumor cells (cytoplasmic staining pattern, is pointed by arrow; B: No staining in normal colon mucosa; C: Weak; D-E: Strong cytoplasmic staining. TNF-α expression is increased in basal tumor cells. In advanced stage tumor TNF-α expression was strong D-E (magnification, × 400).