Increased expression of tumor necrosis factor-α is associated with advanced colorectal cancer stages

Figure 1 Image analysis of immunohistochemistry. Representative images showing how image analysis was performed on cores immunostained for tumor necrosis factor-α. A: A square field is selected; B: A color markup overlay produced by the color deconvolution algorithm reflecting the intensity ranges of the Diaminobenzedine (brown) stain; blue, negative; yellow, weak positive; orange, medium positive; red, strong positive. Scale bar = 100 mm.