Twist1 correlates with poor differentiation and progression in gastric adenocarcinoma via elevation of FGFR2 expression

Figure 3 Twist1 can promote fibroblast growth factor receptor 2 expression in gastric cancer cell lines. A: Twist1 mRNA had a significant association with fibroblast growth factor receptor (FGFR) 2 mRNA level. The correlation between Twist1 and FGFR2 mRNA level was analyzed by Pearson’s test (P < 0.001); B: FGFR2 expression decreased along with Twist1 knockdown in SNU-1 cells. In SNU-1 cells, Twist1 knockdown was accomplished by Twist1 siRNA transfection. Cells were lysed 48 h after siRNA transfection and detected by primary antibody to FGFR2 with immunoblotting; C: FGFR2 expression was elevated by Twist1 overexpression in MKN28 cells. Forty-four hours after transfection with pFLAG-FGFR2 or control vector, MKN28 cells were lysed and FGFR2 expression was detected; D: FGFR2 mRNA level was regulated by Twist1 overexpression or knockdown. Total mRNA was extracted in SNU-1 cells 48 h after transient transfection of Twist1 siRNA or Twist1 vector. FGFR2 mRNA level was evaluated with QPCR. FGFR2 expression decreased when Twist1 was knocked down and increased when Twist1 was overexpressed. ^aP < 0.05, Twist1 siRNA vs scrambled RNA; ^cP < 0.05, Twist1 overexpression vs control vector.