Tricistronic hepatitis C virus subgenomic replicon expressing double transgenes

Figure 2 Response of tricistronic replicon to interferon-α2b and ribavirin. A: Response of tricistronic replicon to increasing doses of interferon (IFN)α2b when transiently transfected. The luciferase activity was substantially reduced in Huh7 cells transiently transfected with tricistronic replicon in a dose-dependent manner. The humanized Renilla luciferase (hRLuc) activity was measured by relative light units and normalized to that of untreated cells; B: Response of tricistronic replicon to different durations of IFNα2b treatment when transiently transfected. The luciferase activity was substantially reduced in Huh7 cells transiently transfected with tricistronic replicon in a time-dependent manner; C: Response of tricistronic replicon to increasing doses of ribavirin when transiently transfected. The luciferase activity was markedly reduced in Huh7 cells transiently transfected with tricistronic replicon in a dose-dependent manner; D: Response of tricistronic replicon to different durations of ribavirin treatment when transiently transfected. The luciferase activity was markedly reduced in Huh7 cells transiently transfected with tricistronic replicon in a time-dependent manner.