PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway

Figure 3 Interference of expression of pre-B-cell leukemia homeobox 3 by shRNAs suppresses cell migration and invasion in vitro. A, B: Expression levels of PBX3 mRNA and protein were significantly decreased after infection with PBX3-shRNA lentivirus compared with the controls as detected by qRT-PCR and Western blot, respectively. Data in (A) are presented as mean ± SD from three independent experiments run in triplicate; C, D: Cell migration and invasion decreased significantly after inhibition of PBX3 expression in LOVO cells (C) and HCT8 cells (D), using a Boyden chamber assay without and with Matrigel. Bar: 50 μm. (E) Quantification of migrating and invading cells through the Transwell membrane are displayed as mean ± SD of three independent experiments in triplicate. P-values are calculated using Student’s t test.