PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway

Figure 2 Ectopic expression of pre-B-cell leukemia homeobox 3 promotes cell spreading, migration and invasion. A, B: Relative expression of PBX3 at both mRNA and protein levels was greatly improved in HT-29 and SW480 cells after infection with PBX3-overexpressing lentivirus compared with the corresponding control cells. Ectopic expression of PBX3 promoted spreading of HT-29 and SW480 cells, in a wound healing assay (C), increased cell migration and invasion via Boyden chamber assay without and with Matrigel, respectively (D). Horizontal bar: 50 μm; E: Quantification results of migrating and invading cells through the Transwell membrane. Data represent mean ± SD of three independent experiments run in triplicate. P-values are calculated using Student’s t test.