Circulating tumor and cancer stem cells in hepatitis C virus-associated liver disease

Table 1 Primer sequences

Gene	Primers sequences	Annealing temp.(°C)	Base pairs
MAGE1	First round:	60	421
	5’-CGG CCG AAG GAA CCT GAC CCA G-3’
	5’-GCT GGA ACC CTC ACT GGG TTG CC-3’
	Second round (nested):	60	299
	5’-ACA GAG GAG CAC CAA GGA GAA G-3’
	5’-AGT TGA TGG TAG TGG GAA AGG C-3’
MAGE3	First round:	60	725
	5’-TGG AGG ACC AGA GGC CCC C-3’
	5’-GGA CGA TTA TCA GGA GGC CTG C-3’
	Second round (nested):	60	371
	5’-CGG AGG AGC ACT GAA GGA GAA G-3’
	5’-CCT CCT CTT CTT CGT TGC TGG-3’
CK-19	5’-CCC GCG ACT ACA GCC ACTA-3’	60	745
	5’-GCT CAT GCG CAG AGC CT-3’
CD133	P1: 5’-AAT TCT AAT ACG ACT CAC TAT AGG GAA GAA CAG GGA TGA TGT TGG GTC TCA-3’
	P2: 5’-TTT CAA GGA CTT GCG AAC TCT CTT GA-3’
	MB: 5’-CGA TCC AAG GAC AAG GCG TTC ACA GGA TCG-3’
Telomerase	5’-TGA CAC CTC ACC TCA CCC AC-3’	60	96
	5’-CAC TGT CTT CCG CAA GTT CAC-3’
β-actin	5’-ACA CTG TGC CCA ACG AGG-3’	56	540
	5’-AGG GGC CGG TCA TAC T-3’'