Proinflammatory effects and molecular mechanisms of interleukin-17 in intestinal epithelial cell line HT-29

Figure 1 Chemokine expression when HT-29 cells were cultured with interleukin-17 and/or tumor necrosis factor-α. When interleukin (IL)-17 and tumor necrosis factor (TNF)-α were used separately, the expression levels of CXCL1, CXCL2, CXCL5, CXCL6, IL-8 and CCL20 in HT-29 cells were comparatively low. However, when IL-17 and TNF-α were used together, the expression levels became strongly upregulated; A: IL-17 + TNF-αvs IL-17, t = 30.424, P = 0.000; IL-17 + TNF-αvs TNF-α, t = 30.438, P = 0.000; B: IL-17 + TNF-αvs IL-17, t = 35.273, P = 0.000; IL-17 + TNF-αvs TNF-α, t = 35.125, P = 0.000; C: IL-17 + TNF-αvs IL-17, t = 85.718, P = 0.000; IL-17 + TNF-αvs TNF-α, t = 94.199, P = 0.000; D: IL-17 + TNF-αvs IL-17, t = 50.165, P = 0.000; IL-17 + TNF-αvs TNF-α, t = 50.224, P = 0.000; E: IL-17 + TNF-αvs IL-17, t = 54.585, P = 0.000; IL-17 + TNF-αvs TNF-α, t = 44.036, P = 0.000; F: IL-17 + TNF-αvs IL-17, t = 56.272, P = 0.000; IL-17 + TNF-αvs TNF-α, t = 57.410, P = 0.000. IL-8: Interleukin-8.