Claudin 1 mediates tumor necrosis factor alpha-induced cell migration in human gastric cancer cells

Figure 1 Claudin 1 controls cell proliferation in MKN28 cells. A: Claudin 1 siRNA effectively reduced the mRNA levels of claudin 1 in MKN28 cells. Mean ± SE. n = 3. ^aP < 0.05 vs control siRNA; B: Western blotting revealed that claudin 1 siRNA reduced the protein levels of claudin 1 in MKN28 cells; C: Downregulation of claudin 1 inhibited the proliferation of MKN28 cells. The number of cells was counted 48 h after siRNA transfection. Mean ± SE. n = 3. ^cP < 0.05 vs control siRNA; D: A second independent claudin 1 siRNA also reduced the mRNA levels of claudin 1 in the MKN28 cells. Mean ± SE. n = 3. ^eP < 0.05 vs control siRNA; E: A second independent claudin 1 siRNA also inhibited the proliferation of MKN28 cells. The number of cells was counted 48 h after siRNA transfection. Mean ± SE. n = 3. ^gP < 0.05 vs control siRNA.