Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis

Figure 4 p62 expression leads to increased iron accumulation and reactive oxygen species production. A, B: Representative paraffin-embedded liver sections stained with Prussian blue for iron accumulation from animals fed the respective diet for four weeks (original magnification × 200 and × 500 for inserts) (A) with the corresponding hepatocellular iron score (B) for all time points (for scoring, see supplement S1); C: Hepatic thiobarbituric acid reactive substances (TBARS) were measured to indicate lipid peroxidation and are presented as the mean ± SEM (n = 10-12). tg: Transgenic; wt: Wild-type; ctrl: Control; MCD: Methionine and choline deficient.