Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis

Figure 1 p62 expression amplifies inflammation. A: Immunofluorescent staining with anti-nuclear factor kappa B (NF-κB)-p65 (red, left panel), 4’,6-diamidino-2-phenylindole (DAPI) for nuclei (blue, middle panel), and merge (right panel) shows activation of NF-κB after four weeks on the methionine-choline deficient (MCD) diet through p65 translocation to the nucleus (white arrows) (original magnification: × 400); B-E: Gene expression analysis from quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) of NF-κB target genes with tumor necrosis factor (TNF) (B), inducible nitric oxide synthase 2 (NOS2) (C), prostaglandin-endoperoxide synthase 2 (PTGS/COX2) (D), and interleukin 1B (IL-1B) (E) from whole livers are expressed as a ratio against the housekeeping gene, 18S. Data are presented as the mean ± SEM (n = 10-12). ctrl: Control; wt: Wild type; tg: Transgenie.