Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis

Table 5 Gas-chromatography mass-spectrometry fatty acid analyses of mice fed the methionine-choline deficient or control diet for two weeks

Fatty acid	ctrl wt	ctrl tg	P value1	MCD wt	MCD tg	P value2	P value3
14:0	0.25 ± 0.04	0.25 ± 0.05	0.970	0.24 ± 0.04	0.43 ± 0.04	0.003	0.005
15:0	0.01 ± 0.003	0.01 ± 0.003	0.113	0.02 ± 0.01	0.05 ± 0.01	0.035	0.002
16:0	17.23 ± 0.79	16.08 ± 1.43	0.623	17.16 ± 1.63	23.85 ± 1.65	0.006	0.0033
16:1	1.69 ± 0.29	1.51 ± 0.30	0.570	0.57 ± 0.10	1.13 ± 0.12	0.005	0.138
17:0	0.11 ± 0.01	0.06 ± 0.01	0.021	0.23 ± 0.03	0.34 ± 0.03	0.010	0.0001
17:1	0.02 ± 0.01	0.01 ± 0.01	0.046	0.003 ± 0.003	0.01 ± 0.01	0.514	0.117
18:0	12.64 ± 0.57	9.41 ± 0.81	0.006	13.08 ± 0.97	18.15 ± 1.00	0.0014	0.0009
18:1	20.13 ± 2.51	17.98 ± 2.07	0.678	24.11 ± 1.28	39.66 ± 3.27	0.001	0.0009
18:2	17.88 ± 0.87	16.42 ± 1.60	0.241	23.90 ± 2.15	36.28 ± 6.34	0.040	0.004
18:3	0.23 ± 0.05	0.27 ± 0.10	0.571	1.50 ± 0.28	2.99 ± 0.46	0.003	0.00009
20:0	0.22 ± 0.05	0.10 ± 0.03	0.045	0.14 ± 0.03	0.33 ± 0.05	0.0007	0.138
20:1	0.40 ± 0.03	0.30 ± 0.04	0.045	0.81 ± 0.14	1.99 ± 0.27	0.002	0.00009
20:2	0.45 ± 0.04	0.53 ± 0.06	0.385	1.67 ± 0.17	2.94 ± 0.25	0.0006	0.00009
20:3	1.09 ± 0.09	0.89 ± 0.14	0.186	2.87 ± 0.37	5.15 ± 0.44	0.0006	0.00009
20:4	13.69 ± 0.58	10.37 ± 0.80	0.011	16.49 ± 1.15	23.20 ± 1.81	0.006	0.00015
22:0	0.59 ± 0.11	0.29 ± 0.07	0.031	0.31 ± 0.03	0.50 ± 0.03	0.0007	0.921
22:1	0.01 ± 0.003	0.003 ± 0.003	0.387	0.01 ± 0.01	0.04 ± 0.01	0.091	0.129
22:4	0.57 ± 0.05	0.51 ± 0.08	0.273	4.24 ± 0.48	6.25 ± 0.56	0.0036	0.00009
22:6	4.72 ± 0.28	3.82 ± 0.29	0.054	12.08 ± 0.90	14.06 ± 1.05	0.214	0.00009
23:0	0.08 ± 0.01	0.04 ± 0.004	0.0003	0.08 ± 0.01	0.10 ± 0.01	0.194	0.223
24:0	0.42 ± 0.02	0.31 ± 0.02	0.003	0.45 ± 0.02	0.63 ± 0.05	0.0013	0.0009

¹P value of the comparison between ctrl wt and ctrl tg;

²P value of the comparison between MCD wt and MCD tg;

³P value of the comparison between ctrl wt and MCD tg; Values (μg/mg dry liver tissue) are expressed as the mean ± SEM. Liver tissues were lyophilized and analyzed by gas-chromatography mass-spectrometry. tg: Transgenic; wt: Wild-type; ctrl: Control; MCD: Methionine-choline deficient.