Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis

Table 4 Liver weight and serum parameters of p62 transgenic and wild-type mice fed a methionine-choline deficient diet or control diet for two and four weeks

	2 wk				4 wk
	ctrl		MCD		ctrl		MCD
	wt	tg	wt	tg	wt	tg	wt	tg
Number of animals	10	10	12	12	10	10	12	12
Relative liver weight (% of body weight)	4.8 ± 0.1	4.6 ± 0.1	3.4 ± 0.1c	4.0 ± 0.2ace	4.2 ± 01	4.1 ± 0.1	3.4 ± 0.1c	3.5 ± 0.2ce
Serum ALT (U/L)	289 ± 83	233 ± 20	418 ± 36c	469 ± 73ce	189 ± 38	222 ± 25	235 ± 40	209 ± 46
Serum AST (U/L)	1568 ± 224	1535 ± 162	2404 ± 125c	2544 ± 207ce	1845 ± 204	1712 ± 253	2813 ± 286c	3057 ± 346c
Serum triglycerides (mg/dL)	244 ± 19	219 ± 17	107 ± 5c	128 ± 11ce	211 ± 18	203 ± 21	95 ± 7c	106 ± 5ce
Serum HDL (mg/dL)	94 ± 6	98 ± 4	24 ± 2c	21 ± 4ce	112 ± 8	119 ± 8	15 ± 2c	18 ± 2ce
Serum glucose (mg/dL)	234 ± 27	179 ± 15	67 ± 10c	59 ± 7ce	193 ± 13	253 ± 36	59 ± 7c	40 ± 5ace

^aP < 0.05 vs wild-type;

^cP < 0.05 vs control diet;

^eP < 0.05 vs wild-type on control diet; Values are expressed as the mean ± SEM; tg: Transgenic; wt: Wild-type; ctrl: Control; MCD: Methionine-choline deficient.