Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis

doi:10.3748/wjg.v20.i47.17839

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 21, 2014; 20(47): 17839-17850
Published online Dec 21, 2014. doi: 10.3748/wjg.v20.i47.17839

Table 2 Primer sequences for real-time reverse transcription-polymerase chain reaction

mRNA	Accession No.	Sense primer, 5'→3’	Antisense primer, 5'→3’
18S	NR_003278.1	GTAACCCGTTGAACCCCATT	CCATCCAATCGGTAGTAGCG
PPARa	NM_001113418.1	CCTTCCCTGTGAACTGACG	CCACAGAGCGCTAAGCTGT
IL-1B	NM_008361.3	GAGAGCCTGTGTTTTCCTCC	GAGTGCTGCCTAATGTCCC
TNF	NM_013693.2	CCATTCCTGAGTTCTGCAAAGG	AGGTAGGAAGGCCTGAGATCTTATC
HMGCR	NM_008255.2	ATCCAGGAGCGAACCAAGAGAG	CAGAAGCCCCAAGCACAAAC
SCD1	NM_009127.4	AGATCTCCAGTTCTTACACGACCAC	CTTTCATTTCAGGACGGATGTCT
CPT1a	NM_013495.2	CTCAGTGGGAGCGACTCTTCA	GGCCTCTGTGGTACACGACAA
NOS2	NM_010927.3	CTCACTGGGACAGCACAGAA	GATGTGGCCTTGTGGTGAA
PTGS/COX2	XM_192868	TGACCCCCAAGGCTCAAATAT	TGAACCCAGGTCCTCGCTTA
FASN	NM_007988.3	GGCTGCTACAAACAGACCAT	CACGGTAGAAAAGGCTCAGT
SREBF2	NM_033218.1	ACCTAGACCTCGCCAAAGGT	CGGATCACATTCCAGGAGA

PPARa: Peroxisome proliferator-activated receptor a; IL-1B: Interleukin 1B; TNF: Tumor necrosis factor; HMGCR: 3-hydroxy-3-methyl-glutaryl-CoA reductase; SCD1: Stearoyl-CoA desaturase 1; CPT1a: Carnitine palmitoyl transferase 1a; NOS2: Nitric oxide synthase 2; PTGS/COX2: Prostaglandin-endoperoxide synthase 2; FASN: Fatty acid synthase; SREBF2: Sterol regulatory element binding transcription factor 2.

Citation: Simon Y, Kessler SM, Gemperlein K, Bohle RM, Müller R, Haybaeck J, Kiemer AK. Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis. World J Gastroenterol 2014; 20(47): 17839-17850
URL: https://www.wjgnet.com/1007-9327/full/v20/i47/17839.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i47.17839