Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis

Table 1 Antibody dilution, unmasking, incubation time, temperature, and immunodetection used for immunofluorescence

Antibody (source)	Unmasking of antigens	Dilution	Incubation	Detection system
NF-κB p65 (Neomarkers, United States)	Citrate buffer pH 6.0, 95 °C, 10 min, water bath	1:1000	18 h at 4 °C	IF with Alexa Fluor 546 (Invitrogen, Germany) as secondary antibody

NF-κB: Nuclear factor kappa B; IF: Immunofluorescence.