Hirschsprung’s disease in twin to twin transfusion syndrome: A case report

Figure 2 Histological and immunohistochemical features of the rectal biopsy specimen. A: Increased numbers of nerve bundles can be seen in the submucosal layer (HE, × 200); B: High-powered magnification of the submucosal nerve plexus shows no ganglion cells (HE, × 400). Immunohistochemically; S-100 (× 200) (C) and bcl-2 (× 200) (D) staining show no ganglion cells in the submucosal nerves. HE: Hematoxylin-eosin staining.