miR-374b-5p suppresses RECK expression and promotes gastric cancer cell invasion and metastasis

Figure 3 Regulation of RECK expression in MGC-803 cells by miR-374b-5p. A: The miR-374b-5p binding site within the 3′ UTR of human (hsa) RECK cDNA was predicted with PicTar, TargetScan and miRanda software; B: Cells were co-transfected with RECK 3’UTR-pmirGLO and a plasmid containing either miR-374b-5p or miR-NC plasmid, ^aP < 0.05 vs miR-NC (n = 3); C: Western blot of proteins; D: qRT-PCR from cells transfected for 48 h with anti-miR-374b-5p or a negative control vector, or treated with phosphate-buffered saline (untreated), or transfection reagent only; (n = 3), ^cP < 0.05 vs Anti-miR-374b-5p.