miR-374b-5p suppresses RECK expression and promotes gastric cancer cell invasion and metastasis

Figure 2 Effects of miR-374b-5p on MGC-803 cell metastasis and invasion. MGC-803 cells were transfected with a miR-374b-5p antisense oligonucleotide or negative control-oligo vector, or treated with phosphate-buffered saline (untreated), or transfection reagent only. A: Cells were subjected to a transwell invasion assay and detected at 48 h after seeding (left: Coomassie Brilliant Blue staining, × 200; right, quantification); B: Wound margin distance was measured after 48 h in a scratch assay (Coomassie Brilliant Blue staining, × 40); C: Absorbances in a cell counting kit-8 assay 96 h after transfection; (n = 3), ^aP < 0.05 vs Anti-miR-374b-5p.