Pea3 expression promotes the invasive and metastatic potential of colorectal carcinoma

doi:10.3748/wjg.v20.i46.17376

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 14, 2014; 20(46): 17376-17387
Published online Dec 14, 2014. doi: 10.3748/wjg.v20.i46.17376

Table 1 Primer sequences used in quantitative polymerase chain reaction analysis

Target gene	Forward	Reverse
GAPDH	ACCCACTCCTCCACCTTTGA	CATACCAGGAAATGAGCTTGACAA
PEA3	AGGAGACGTGGCTCGCTGA	AACCTAGCTTTCCACAGCCCC
FSP1	TGATCCTGACTGCTGTCATGG	TACTCTTGGAAGTCCACCTCG
TWIST	GACCTCGGGGCCATCCACACC	CGCCGCCGCCGCCACAGC
VIMENTIN	GCGTGACGTACGTCAGCAATATGA	GTTCCAGGGACTCATTGGTTCCTT
SLUG	ACGCCTCCAAAAAGCCAAACTACA	CTTCAGGGCGCCCAGGCTCACATA
SNAIL	AGGCCCTGGCTGCTACAAG	TGCCCTCCCTCCACAGAAAT
ZEB-1	TTGCCGCTGTTGCTGATGTG	GTGCCCTGCCTCTGGTCCTC
E-CADHERIN	CTTCACCGACTTACCTACT	GTGCCATACACTTAATTCTC
MMP1	ATTCTACTGATATCGGGGCTTTGA	ATGTCCTTGGGGTATCCGTGTAG
MMP2	TATTTGATGGCATCGCTCAG	GCCTCGTATACCGCATCAAT
MMP3	ATGCCCACTTTGATGATGATGAAC	CCACGCCTGAAGGAAGAGATG
MMP7	TGCTCACTTCGATGAGGATG	TGGGGATCTCCATTTCCATA
MMP9	TTGACAGCGACAAGAAGTGG	CCCTCAGTGAAGCGGTACAT
MMP10	GAGTGGGGCAGCAAAAGAGGAG	TGGGCCATCAAAAGAGTAAAAGTC
MMP13	CTTCGGCTTAGAGGTGACTGG	TTTTGCCGGTGTAGGTGTAGATAG
MMP14	AGGCCGACATCATGATCTTCTTTG	GGTGGGGTTTTTGGGTTTATCAGG
TIMP1	ACCCCCGCCATGGAGAGTGTC	CCGGAAGAAAGATGGGAGTG
TIMP2	CGCAGCAAACACATCCGTAGAAGG	CCGAGGAGGGGGCCGTGTAGATAA
TIMP3	CCCTTCGGCACGCTGGTCTACA	TCAGCGGGAAGGGAGGGAAGTG
TIMP4	CGTCGGGGCGGGATTGG	ACTGCTTCTGGCTGTTGGCTTCTA

Sequences were designed with Primer 3 software (http://primer3.sourceforge.net/) or with the DNASTAR Lasergene Suite (Madison, WI, United States). Sequences were checked against the human transcript database via PrimerBlast (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) for cross-amplification of unintended transcripts. MMP: Matrix metalloproteinase; TIMP: Tissue inhibitor of metalloproteinase.

Citation: Mesci A, Taeb S, Huang X, Jairath R, Sivaloganathan D, Liu SK. Pea3 expression promotes the invasive and metastatic potential of colorectal carcinoma. World J Gastroenterol 2014; 20(46): 17376-17387
URL: https://www.wjgnet.com/1007-9327/full/v20/i46/17376.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i46.17376