CYLD deletion triggers nuclear factor-κB-signaling and increases cell death resistance in murine hepatocytes

Figure 5 Nuclear factor-κB and survivin inhibition sensitized primary murine hepatocytes towards receptor-mediated cell death. A: Western blot analysis for nuclear factor (NF)-κB regulated gene expression after tumor necrosis factor (TNF)-R (left panel) and CD95-R (right panel) triggering of CYLD^-/- primary murine hepatocytes (PMH) 4 h pre-incubated with BAY 11-7085; B: 24 h treatment of WT and CYLD^-/- PMH with increasing concentrations of BAY 11-7085 (left panel) and YM155 (right panel); C: WT and CYLD^-/- PMH were pre-incubated with 10 μmol/L BAY-11 7085 for 4 h. Afterwards PMH were treated for 24 h in combination with TNF-α or Jo2 as indicated (upper panel). Pre-incubation of CYLD^-/- PMH with 20 μmol/L BAY-11 7085 and following TNF-α or Jo2 treatment (lower panel); D: Western blot analysis for survivin expression in WT and CYLD^-/- PMH 24 h after treatment with YM155 as indicated (upper panel). WT and CYLD^-/- PMH were pre-incubated with 10 µmol/L YM155 for 4 h. Afterwards PMH were treated for 24 h in combination with TNF-α or Jo2 as indicated (lower panel). DMSO was used as vehicle. Values represent the mean ± SD. ^aP < 0.05, CYLD^-/-vs control group; ^bP < 0.01, WT vs CYLD^-/-; ^dP < 0.01, WT vs control group; ^fP < 0.01, CYLD^-/-vs control group.