Estradiol agonists inhibit human LoVo colorectal-cancer cell proliferation and migration through p53

Figure 4 17β-estradiol and/or ER selective agonistsdown-regulate the protein levels of u-PA, t-PA and MMP-9 inLoVocells via p53. A: LoVo cells were treated with E2 or various concentrations (10^-10 mol/L, 10^-9 mol/L and 10^-8 mol/L) of PPT or DPN for 24 h; B: LoVo cells were pretreated with various concentrations (10^-10 mol/L, 10^-9 mol/L and 10^-8 mol/L) of a p53 inhibitor for 1 h, followed by E2 administration for 24 h; C: LoVo cells were pretreated with vehicle, the ER antagonist ICI 182780, or a p53 inhibitor for 1 h, followed by E2 administration for 24 h. Cells were harvested and examined by Western blotting. A β-actin or α-tubulin standard was used as a loading control for all proteins. All experiments were repeated twice with identical results.