Estradiol agonists inhibit human LoVo colorectal-cancer cell proliferation and migration through p53

Figure 3 17β-estradiol and ER selective agonists impair the Wnt-β-catenin signaling pathway in human LoVo colorectal cancer cells. LoVo cells were treated with E2 (10^-8 mol/L) or various concentrations (10^-10 mol/L, 10^-9 mol/L and 10^-8 mol/L) of PPT (A) or DPN for 24 h (B). Cells were harvested and measured by Western blotting. An α-tubulin standard was used as a loading control for all proteins. All experiments were repeated twice with identical results.