Phosphatidylinositol 3-kinase CB association with preoperative radiotherapy response in rectal adenocarcinoma

Figure 3 Both at mRNA (A) and protein (B) levels, PIK3CB expression was significantly upregulated in all four colon cancer cells tested (HCT-116, HT29, LoVo, and LS174T) after in vitro 6-Gy irradiation for 1-12 d. In vitro clonogenic formation assay after exposure to 6-Gy ionizing radiation of HCT-116, HT-29, LoVo and LS174T colorectal cancer cells, with or without 100 μmol/L TGX221 (a PIK3CB-specific inhibitor). Each experiment was performed in triplicate and with three repeats for each group. Error bars represent ± 1 SEM. R: Radiation.