GW4064, a farnesoid X receptor agonist, upregulates adipokine expression in preadipocytes and HepG2 cells

Figure 3 GW4064 (5 μmol/L) stimulates adiponectin receptor 2 (A) and long form of the leptin receptor (B) mRNA expression in HepG2 cells in a time-dependent manner. HepG2 cells were serum-deprived 24 h before GW4064 was added. Total RNA was extracted and subjected to fluorescent real-time PCR as described in Section 2. AdipoR2 and OB-Rb mRNA levels were normalized to GAPDH expression and compared to cells at 0 h (100%). Data represent mean ± SD of three independent experiments. ^aP < 0.05 vs the cells at 0 h. Error bars = mean ± SD. AdipoR2: Adiponectin receptor 2; OB-Rb: Long form of the leptin receptor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.