Esophageal Helicobacter pylori colonization aggravates esophageal injury caused by reflux

Figure 5 Ki-67 expression and TdT-mediated dUTP nick-end labeling assay in rat esophagus. A: Immunohistochemistry (IHC) scoring of Ki-67 expression in the esophagus; B: The scoring of apoptotic cells in the esophagus as determined by TdT-mediated dUTP nick-end labeling (TUNEL) assay; C: IHC staining of Ki-67-positive cells and TUNEL-positive apoptotic cells. a, e: Normal esophagus mucosa; b, f: Esophagitis; c, g: Barrett’s esophagus; d, h: Esophageal adenocarcinoma; a-d: IHC staining of Ki-67; e-h: apoptotic cells stained by TUNEL. All of the samples were viewed under 200 × magnification. ^bP < 0.01 vs pseudo-operation and pseudo-operation with Helicobacter pylori (H. pylori) infection groups; ^cP < 0.05, ^dP < 0.01 vs esophagogastroduodenal anastomosis (EGDA) group.