Chunggan extract, a traditional herbal formula, ameliorated alcohol-induced hepatic injury in rat model

Figure 3 Changes in hepatofibrosis-associated cytokines in protein and gene expression. Rats were orally administered 30% alcohol (10 mL/kg) with or without Chunggan extract (CGX; 100 or 200 mg/kg) for 4 wk. The levels of transforming growth factor-beta (TGF-β), platelet-derived growth factor-BB (PDGF-BB), connective tissue growth factor (CTGF), and interferon-gamma (IFN-γ) were determined in liver homogenates by enzyme-linked immunosorbent assays (A), and their gene expressions were determined by real-time polymerase chain reactions (B). Data are expressed as means ± SD (n = 6-9). ^aP < 0.05 vs normal group, ^bP < 0.01 vs normal group, ^cP < 0.05 vs control group, ^dP < 0.01 vs control group. ¹Only TGF-β was expressed as ng/100 μg protein. Others were according to the mg/protein, but TGF-β1 was only according to the 100 μg/mg protein.