Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity

Figure 4 Palmitoylation of Wnt2B and expression levels of acyl-CoA synthetase 5, Wnt2B, and β-catenin in colon carcinoma cell lines of different malignancy statuses. A: HT29, CaCo₂, SW480, and HCT116 were treated with palmostatin B, mitochondria isolated, Wnt2B immunoprecipitated and palmitoylation was detected via streptavidin antibody in Western blot; B: mRNA expression of acyl-CoA synthetase 5 (ACSL5), Wnt2B, and β-catenin. Realtime PCR with cyclophilin as reference gene. ^aP < 0.05 vs control.