FXR agonist GW4064 alleviates endotoxin-induced hepatic inflammation by repressing macrophage activation

Figure 4 GW4064 represses lipopolysaccharide-induced proinflammatory cytokine expression in RAW 264. 7 cells. RAW 264.7 cells were cultured in Dulbecco's Modified Eagle Media with 10% FBS, treated with GW4064 for 24 h, then treated with lipopolysaccharide (LPS) for 6 h. Expression of Mcp1, Il1b, Il6, and Ifng in RAW 264.7 cells was evaluated by real-time polymerase chain reaction. ^aP < 0.05, ^bP < 0.01 vs LPS group.