Production of corticotropin-releasing factor and urocortin from human monocyte-derived dendritic cells is stimulated by commensal bacteria in intestine

Figure 3 Production of corticotropin-releasing factor in dendritic cells stimulated with commensal bacteria. Human monocyte-derived DCs (MoDCs) from 5 healthy donors (①, ②, ③, ④, and ⑤) were stimulated with A: Bacteroides vulgatus (B. vulgatus); B: Fusobacterium varium (F. varium); C: Escherichia coli (E. coli); D: Clostridium clostridioforme (C. clostridioforme); E: Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus) for 24 h, after which the production of corticotropin-releasing factor (CRF) was analyzed. Supernatants from F: human MoDCs alone and G: commensal bacteria alone were used as controls; H: The production of CRF in human MoDCs stimulated with B. vulgatus was compared with that in human MoDCs stimulated with F. varium. The results are expressed as the mean ± SD from 5 healthy donors. ^bP < 0.01, MoDCs + B. vulgatus vs MoDCs s + F. varium.