Endoscopic ultrasound guided fine needle aspiration and useful ancillary methods

doi:10.3748/wjg.v20.i39.14292

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Oct 21, 2014 (publication date) through Nov 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 21, 2014; 20(39): 14292-14300
Published online Oct 21, 2014. doi: 10.3748/wjg.v20.i39.14292

Table 1 Possible methods of sample processing

Sample types	Ancillary methods
Direct cytological smears	Cytomorphological diagnostic evaluation
	ICC staining
	Molecular testing (FISH)
	Excellent source for isolation of good quality DNA[39,40]
Cell blocks (formalin-fixed, paraffin-embedded tissue)	IHC
	Various molecular tests
	Tissue storage
Touch imprint slides (tumor cell rich slides made by imprinting the tumor)	IHC
	Molecular testing (FISH)
Fresh aspirated specimens (especially of lymph nodes)	Flow cytometry - immunophenotyping
	RNA based gene expression studies
	DNA based studies[41]
Fresh frozen (cryopreserved) FNA specimens	RNA based gene expression studies
Fresh frozen (cryopreserved) FNA specimens	DNA based studies[42]
FTA cards (filter paper)	Effective RNA and DNA preservation
FTA cards (filter paper)	Not suitable for morphology[43]
Formalin fixed paraffin embedded core tissue	Histological morphologic evaluation
Formalin fixed paraffin embedded core tissue	IHC and molecular studies

FISH: Fluorescence in situ hybridization; FNA: Fine needle aspiration; ICC: Immunocytochemistry; IHC: Immunohistochemistry.

Citation: Tadic M, Stoos-Veic T, Kusec R. Endoscopic ultrasound guided fine needle aspiration and useful ancillary methods. World J Gastroenterol 2014; 20(39): 14292-14300
URL: https://www.wjgnet.com/1007-9327/full/v20/i39/14292.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i39.14292