Distribution of the P2X2 receptor and chemical coding in ileal enteric neurons of obese male mice (ob/ob)

Figure 1 Examination of immunoreactivity for the P2X2 receptor, nitric oxide synthase and choline acetyl transferase. Immunoreactivity for the P2X2 receptor (A, G), NOS (B, E), and ChAT (H, K) was examined in myenteric plexus neurons from the control group (CG) (A-C, G-I) and the obese ob/ob group (OG) (D-F, J-L). Merged images are show in C, F, I and L. Single arrows show the double-labeled P2X2 receptor and NOS-ir neurons in the CG (A-C) and OG (D-F). Double-labeling for P2X2 and ChAT in CG (G-I) and OG (J-L) neurons is indicated by arrows. Note that the P2X2 receptor did not label myenteric neurons in the OG (D, J). NOS: Nitric oxide synthase; ChAT: Choline acetyl transferase.