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©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Sep 28, 2014; 20(36): 13119-13126
Published online Sep 28, 2014. doi: 10.3748/wjg.v20.i36.13119
Published online Sep 28, 2014. doi: 10.3748/wjg.v20.i36.13119
Figure 7 Scheme of reversible immortalization.
The primary hepatocytes were immortalized by transfer of SV40Tag. After expansion of the immortalized cells, Cre/loxP recombination was performed to remove the oncogene (SV40T) and cells reverted to their preimmortalized state.
- Citation: Meng FY, Liu L, Yang FH, Li CY, Liu J, Zhou P. Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination. World J Gastroenterol 2014; 20(36): 13119-13126
- URL: https://www.wjgnet.com/1007-9327/full/v20/i36/13119.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i36.13119