Helicobacter pylori infection, gastrin and cyclooxygenase-2 in gastric carcinogenesis

Figure 4 Mature amidated gastrin (G17) induces cyclooxygenase-2 expression in gastric cancer cells. Western blot analysis was performed on cell lysates (30 μg) from SGC-7901 and MKN-45 cells cultured for 24 h with increasing concentrations of G17 (A), or with G17 at 10 nmol/L for the indicated time points (B). The top panels show a representative immunoblot of six separate experiments undertaken. The histograms at the bottom represent the relative expression of cyclooxygenase-2 (COX-2) proteins compared with tubulin. Data represent the mean ± SD of six independent experiments. ^aP < 0.05, ^bP < 0.01 vs 0 nmol/L or 0 h. Reproduced from Ref [74] with permission.