Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination

doi:10.3748/wjg.v20.i36.13119

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Sep 28, 2014 (publication date) through Feb 18, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 28, 2014; 20(36): 13119-13126
Published online Sep 28, 2014. doi: 10.3748/wjg.v20.i36.13119

Table 1 Primers used in reverse transcription-polymerase chain reaction in this study

Gene	Forward (Tm)	Reverse (Tm)	Product size
SV40T	CAGGCATAGAGTGTCTGC	CAACAGCCTGTTGGCATATG	422 bp
Human ASGPR	TAGGAGCCAAGCTGGAGAAA	ACCTGCAGGCAGAAGTCATC	495 bp
Human GST-II	GCCCTACACCGTGGTCTATT	GGCTAGGACCTCATGGATCA	496 bp
Human GS	ATGCTGGAGTCAAGATTGCG	TCATTGAGAAGACACGTGCG	535 bp
Human Albumin	AAACCTCTTGTGGAAGAGCC	CAAAGCAGGTCTCCTTATCG	576 bp
Human HBCF-X	GTGCATGGAAGAGACCTGCT	GAAGTCAAGCAGGTCGAAGG	493 bp
Human β-actin	TGACGGGGTCACCCACACTGTGCCCATCTA	AGAAGCATTTGCGGTGGACGATGGAGGG	610 bp

Human ASGPR: Human asialoglycoprotein receptor; GST-II: Glutathione-S-transferase II; GS: Glutamine synthetase; HBCF-X: Human blood coagulation factor X.

Citation: Meng FY, Liu L, Yang FH, Li CY, Liu J, Zhou P. Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination. World J Gastroenterol 2014; 20(36): 13119-13126
URL: https://www.wjgnet.com/1007-9327/full/v20/i36/13119.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i36.13119