Osteopontin is an important mediator of alcoholic liver disease via hepatic stellate cell activation

Table 4 Composition of buffers used in protein extraction and expression studies

Buffer	Composition
RIPA	0.5% (w/v) sodium deoxycholate, 150 mmol/L NaCl, 50 mmol/L Tris pH 8.0, 20 mmol/L NaF, 10% (v/v) glycerol, 5.0 mmol/L EDTA pH = 8.0, 0.1% (w/v) SDS, 1.0% (v/v) Igepal CA-630 Electrophoresis reagent, 1 × phosSTOP, 1 × complete protease inhibitor
TBS	50 mmol/L Tris, 150 mmol/L NaCl, pH = 7.5
TBST	0.1% (v/v) Tween 20 in TBS
Horseradish peroxidase inactivation	0.05% (v/v) of sodium azide, 3% (w/v) skim milk and 0.1% (v/v) Tween 20 in TBS