Osteopontin is an important mediator of alcoholic liver disease via hepatic stellate cell activation

Table 3 Primer sequences for human (h-) and mouse (m-) genes

Target gene	Forward (5’→3’)	Reverse (5’→3’)	Expected size (bps)	Ref.
OPN and OPN pathway
Total h-OPN	AATGGTGCATACAAGGCCATC	TGTCCTTCCCACGGCTGT	90	NM_000582[1]
h-OPN-C	CTGAGGAAAAGCAGAATG	AATGGAGTCCTGGCTGT	149	[40]
h-CD44v6	GCTTTCAATAGCACCTTGCC	GTTGCCAAACCACTGTTCCT	867	[41]
h-αvβ3-integrin	TTGTTGCTACTGGCTGTTTTG	TCCCTTTCTTGTTCTTCTTGAG	89	NM_002210
m-OPN	CAGCCTGCAAGATCCTA	GCGCAAGGAGATTCTGCT TCT	71	[7]
m-αvβ3-integrin	GTAATCGAGATGCCCCAGAG	CTTCCATCCAGGGCAATATG	142	[42]
m-CD44	TCTGCCATCTAGCACTAAGAGC	GTCTGGGTATTGAAAGGTGTAGC	106	[43]
Fibrogenesis and ECM
h-TGFβ	CCACAACGAAATCTATGAC	CGGTGACATCAAAAGATAAC	260	NM_000660
m-TGF-β	CCTTCCTGCTCCTCATGGCCA	GTCCTTCCTAAAGTCAATGTA	149	[44]
h-Collagen 1α1	GATTCCCTGGACCTAAAGGTGC	AGCCTCTCCATCTTTGCCAGCA	106	NM_000088.3
m-Collagen 1 (Col1)	GAGCGGAGAGTACTGGATCG	GCTTCTTTTCCTTGGGGTTC	158	[45]
h-Collagen 4α4	CAGGCTCAACTGGTCTAAGAGG	AGGTGGACCAAAGTGACTGGCA	157	NM_000092.4
h-MMP2	ATGACAGCTGCACCACTGAG	ATTTGTTGCCCAGGAAAGTG	164	NM_004530.2
h-MMP3	GCAGTTTGCTCAGCCTATCC	AGTGTCGGAGTCCAGCTTC	194	NM_002422.3
h-MMP9	ACGCCGCTCACCTTCACT	AGGAGGAAAGGCGTGTGC	111	NM_004994
m-MMP7	GCGGAGATGCTCACTTTGACA	ATTCATGGGTGGCAGCAAAC	87	[46]
m-MMP9	CCCACATTTGACGTCCAGAGAAGAA	GTTTTTGATGCTATTGCTGAGATCCA	205	[46]
m-α-sma	AACAGGAATACGACGAAG	CAGGAATGATTTGGAAAGGA	134	[47]
Plasmin-Plasminogen system
h-μPA	CACACACTGCTTCATTGATTAC	TTGGTGGTGACTTCAGAGCCG	409	NM_002658
h-PLG (Plasminogen)	GCAGTGGAGAAAACTATGACGGC	GAAGATGGTGGAGGTGTTGTGC	244	NM_000301[23]
h-PAI-1	CTTTGGTGAAGGGTCTGCTGTG	TGGGTTTCTCCTCCTGTTGTCAG	183	M16006[23]
m-PLG	GCCCAACCTACCAATGTCTGAAAG	GGTGGAACTGAGGAATCTGACTGG	292	[23]
m-μPA	AGGGTGAGCGCCAATAGCAT	GATACATTCACGTGGAGCAT	530	[48]
m-PAI-1	ATGCCATCTTTGTCCAGCGG	TTGGTATGCCTTTCCACCCAG	151	[23]
Normalising
h-β-actin	TGCCATCCTAAAAGCCAC	TCAACTGGTCTCAAGTCAGTG	289	NM_001101
m-β-actin	AAATCGTGCGTGACATCAAA	AAGGAAGGCTGGAAAAGAGC	178	[49]