Osteopontin is an important mediator of alcoholic liver disease via hepatic stellate cell activation

Table 2 Antibodies for Western blots, immunofluorescence and enzyme-linked immunosorbent assay

Specificity	Host	Isotype	Use	Dilution	Samples	Source (Catalog No.)
Primary antibodies
OPNK20 (SPP1)	Goat	Polyclonal IgG	WB	1:1000	Human, LX2	Santa Cruz, Santa Cruz, CA, United States (sc-10591)
			Blocking	200 ìg/mL
OPN (AF808)	Goat	Polyclonal IgG	WB	1:1000	Mice	RD (AF808)
Capture anti-OPN	Mouse	Monoclonal	ELISA	3 ìg/mL	Human, LX2	RD (MAB14331)
Detection biotynlated anti-OPN	Goat	Polyclonal IgG	ELISA	200 ng/mL	Human, LX2	RD (BAF1433)
OPN-R3 aptamer blocks SPP1 binding to receptors				100 nmol/L	LX2	Duke University, NC, United States
αvβ3-integrin (CD51/61) (ITGAV)	Mouse	Monoclonal	Blocking	10 ìg/mL	LX2	RD, Minneapolis, MN, United States (MAB3050)
CD44v6	Mouse	Monoclonal IgG1	Blcoking	1:1000	LX2	RD (BBA13)
Vimentin	Rat	Monoclonal	IF	1:200	LX2	RD (MAB2105)
Phospho-Akt Ser473 (P-Akt)	Rabbit	Polyclonal	WB	1:1000	LX2, Mice	Cell Signalling Technology (No. 9271)
Total-Akt (pan)	Rabbit	Polyclonal IgG	WB	1:1000	LX2, Mice	Cell Signalling Technology (No. 4691)
Phospho-Erk p44/42 (P-Erk)	Mouse	Monoclonal IgG	WB	1:2000	LX2, Mice	Cell Signalling Technology, Danvers, MA, United States (No. 9106)
Total-Erk (T-Erk)	Rabbit	Polyclonal	WB	1:1000	LX2, Mice	Cell Signalling Technology (No. 9102)
GAPDH	Mouse	Monoclonal	WB	1:200000	Mice	Ambion (AM4300)
Secondary antibodies
Anti-mouse HRP (P0161)				1:10000	Dako Australia Pty Ltd, Campbellfield, VIC, Australia
Anti-rabbit HRP (P0449)				1:7500
Anti-goat HRP (P0448)				1:75000
AlexaFluor 488; AlexaFluor 594				1:400	Molecular probes

WB: Western blots; ELISA: Enzyme-linked immunosorbent assay; IF: Immunofluorescence; OPN: Osteopontin; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HRP: Horseradish peroxidase.