Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice

Figure 4 Assessment of hydrogen peroxide production. A: Mouse colonic epithelial cells were incubated for 30 min with 10 μg/mL LPS, 10 μmol/L DPI, 10 μg/mL LPS + 10 μmol/L DPI or without stimulation; B: Colonic biopsies (10 mg) of DSS-induced colitis mice and control subjects were incubated without stimulation for 30 min. ^aP < 0.05, DSS-induced colitis groups vs stimulated and un-stimulated cell subgroups within control. ^cP < 0.05, untreated vs DPI treated subgroups in the chronic DSS-induced colitis group. ^eP < 0.05, LPS vs LPS + DPI treated cell subgroups in both DSS-induced colitis groups. ^gP < 0.05, untreated vs LPS treated cell subgroups in the acute DSS-induced colitis group. ⁱP < 0.05, DSS-induced colitis groups in the mouse colonic biopsies vs control. Values are mean ± SE. Untreated mouse group: n = 12. Both DSS-induced colitis groups: n = 8.